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- Title
Immunohistochemical (IHC) Overexpression of Succinate Dehydrogenase (SDHB) as Prognostic Factor in Sporadic Pheochromocytomas/Paragangliomas (P/P).
- Authors
Pusceddu, S.; Milione, M.; Mazzaferro, V.; De Braud, F.; Coppa, J.; Procopio, G.; Seregni, E.; Biondani, P.; Damato, A.; Buzzoni, R.
- Abstract
Introduction: Recently, gene mutations (SDH complex) involved in the pathogenesis of familial P/P have been discovered. These mutations are often associated with SDHB activity loss and activation of hypoxia signals. Aim(s): SDHB IHC expression in sporadic P/P with limited or advanced disease. Materials and methods: Eleven P/P treated from 1999 to 2009 were identified: median age 62 yrs (range 34-78), males/females = 4/7.28% were in stage IV, with syncronous and metachronous mts in 33% and 37%, respectively. SDHB IHC expression were carried out in all primary tumor tissues using a semiquantitative scale, considering staining intensity (score 0-3). The staining for SDHB was optimized employing micro-slices of normal myocardial atrial muscle as positive control. So as to ensure antibody specificity, consecutive sections were incubated in the absence of primary antibody. Results: Loss of SDHB expression (0) was found only in 2/11 pts with advanced disease, whilst high positivity (3+), with a clear cytoplasmic mitochondrial reactivity, was found in 100% (3/3) and 40% (2/5) of PIP with synchronous and metachronous liver metastases, respectively. One-third (33%) of non-metastatic pts demonstrated a high positivity. Conclusion: These data, even if on a limited number of cases of sporadic P/P, do not support the association between loss of SDHB and malignancy advocated for familial P/P. On the contrary, our findings could suggest a possible correlation between a higher incidence of SDHB IHC intensity (score 3+) and advanced disease.
- Subjects
IMMUNOHISTOCHEMISTRY; FAMILIAL diseases; SUCCINATE dehydrogenase; PHEOCHROMOCYTOMA; ADRENAL tumors; PARAGANGLIOMA; LIVER cancer
- Publication
Neuroendocrinology, 2012, Vol 96, p12
- ISSN
0028-3835
- Publication type
Article