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- Title
Generation of a monoclonal antibody against the RBD of the Spike protein from SARS CoV-2-.
- Authors
García Cordero, J.; Mendoza-Ramírez, N. J.; Cedillo Barron, L.
- Abstract
The COVID-19 is a disease caused by the SARS CoV-2; virus responsible for millions of deaths worldwide 1. SARS-CoV-2 is an enveloped, positive, and single-stranded RNA virus. Its genome encodes four structural proteins: membrane (M), envelope (E), spike (S), and nucleocapsid (N). The spike glycoprotein (S) protein is comprised of S1 & S2 subunits. These spike protein subunits enable viral attachment by binding to the host cell via ACE-2 (angiotensin converting enzyme-2) receptor (RBD). The Spike protein induce an immune a protective immune response and it has been used as a vaccine target, and for diagnosis, furthermore; an anti-Spike monoclonal antibody is a power tool to evaluate many aspects of virus molecular biology and many aspects of pathogenicity of the SARS CoV-2. In the present work we design express and purify the RBD protein of SARS CoV-2. in mammalian platform, to immunize BALB/c mice for the generation of monoclonal antibodies. After all steps for get monoclonal antibodies. This antibody was able to recognize the viral protein trough different approaches such as ELISA, WB and in immunofluorescence assays using cells Vero infected with the SARS CoV-2 virus. This antibody will provide us with an essential tool for studies on the pathogenicity of the SARS CoV-2.
- Publication
Veterinaria México OA, 2024, Vol 11, p36
- ISSN
2448-6760
- Publication type
Article
- DOI
10.22201/fmvz.24486760e.2024.1305