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- Title
Permissivity of BeWo cells differentiated to syncytiotrophoblasts to human respiratory syncytial virus infection.
- Authors
Cruz Holguín, Victor Javier; López Montesinos, Ángel Guillermo; Moro Huitrón, Luis Antonio Herrera; Ulloa Aguilar, José Manuel; Velázquez Cervantes, Manuel Adrián; García, Mauricio Comas; Gutiérrez, Carlos Cabello; Juarez, Paola Castillo; Sepulveda, Christian García; Cordero, Julio García; Barrón, Leticia Cedillo; Juárez, Moisés León
- Abstract
The Human Respiratory Syncytial Virus (RSV) is one of the leading agents causing respiratory tract infections in children. Transmission of RSV is mainly respiratory. However, vertical transmission from mother to fetus during pregnancy is poorly understood. Until now, in-vitro studies using primary cultures of trophoblasts and cell lines such as BeWo have shown to be permissive to infection by this virus. Also, infection with murine models is able of vertical transmission. Reports in humans have suggested vertical transmission of this virus, which could interfere with fetal programming, thus reducing fetal developmental pathways and modifying postnatal metabolism, making it susceptible to respiratory diseases. For this reason, it is crucial to understand the viral mechanisms during vertical transmission to develop possible treatments for infection by this virus. In this sense, RSV can infect human placental cytotrophoblast (BeWo) cell lines, but the permissiveness of syncytiotrophoblasts (first placental barrier) is unknown. In this work, we developed the differentiation of the BeWo cell line to syncytiotrophoblasts in-vitro, under cAMP treatment. The syncytiotrophoblasts derived from the BeWo line, whose differentiation was evidenced by the expression of SYNCYTIN2 and SIK1 at the mRNA and protein level, as well as the formation of syncytia revealed by Immunofluorescence, also we demonstrated using Immunofluorescence and Western Blot assays, that they are permissive to RSV infection. Additionally, in syncytiotrophoblast, an increase in the expression of the viral receptor for RSV (nucleolin) was demonstrated, which could help us to understand this cell model as a target of RSV infection and vertical transmission.
- Publication
Veterinaria México OA, 2024, Vol 11, p20
- ISSN
2448-6760
- Publication type
Article
- DOI
10.22201/fmvz.24486760e.2024.1305