We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
RNA-Seq time-course analysis of neural precursor cell transcriptome in response to herpes simplex Virus-1 infection.
- Authors
Wood, Joel A.; Chaparala, Srilakshmi; Bantang, Cecilia; Chattopadhyay, Ansuman; Wesesky, Maribeth A.; Kinchington, Paul R.; Nimgaonkar, Vishwajit L.; Bloom, David C.; D'Aiuto, Leonardo
- Abstract
The neurogenic niches within the central nervous system serve as essential reservoirs for neural precursor cells (NPCs), playing a crucial role in neurogenesis. However, these NPCs are particularly vulnerable to infection by the herpes simplex virus 1 (HSV-1). In the present study, we investigated the changes in the transcriptome of NPCs in response to HSV-1 infection using bulk RNA-Seq, compared to those of uninfected samples, at different time points post infection and in the presence or absence of antivirals. The results showed that NPCs upon HSV-1 infection undergo a significant dysregulation of genes playing a crucial role in aspects of neurogenesis, including genes affecting NPC proliferation, migration, and differentiation. Our analysis revealed that the CREB signaling, which plays a crucial role in the regulation of neurogenesis and memory consolidation, was the most consistantly downregulated pathway, even in the presence of antivirals. Additionally, cholesterol biosynthesis was significantly downregulated in HSV-1-infected NPCs. The findings from this study, for the first time, offer insights into the intricate molecular mechanisms that underlie the neurogenesis impairment associated with HSV-1 infection.
- Subjects
INDUCED pluripotent stem cells; HUMAN herpesvirus 1; HERPES simplex virus; NEURAL stem cells; CENTRAL nervous system; DEVELOPMENTAL neurobiology
- Publication
Journal of NeuroVirology, 2024, Vol 30, Issue 2, p131
- ISSN
1355-0284
- Publication type
Article
- DOI
10.1007/s13365-024-01198-8