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- Title
Correlation between 5-HT Content and Uptake Site Density following ( S)-MDMA and Dexfenfluramine-induced Depletion, and with Neuroprotection by the Glycine Site-specific NMDA Antagonist ACEA 1021.
- Authors
RUSSELL, B. R.; LAVERTY, R.
- Abstract
A bstract: 3,4-Methylenedioxymethamphetamine (MDMA) and fenfluramine are amphetamine analogues that both cause long-term depletion of serotonin (5-HT) and 5-HT uptake sites in brain tissue. Depletion caused by these amphetamines is commonly measured by labeling 5-HT uptake sites using 3H-paroxetine combined with autoradiography or, alternatively measuring the concentration of 5-HT in tissue using high-performance liquid chromatography (HPLC). A close correlation between the 5-HT concentration measured in micropunch samples and the density of 3H-paroxetine-labeled 5-HT uptake sites measured in corresponding 20 μm coronal slices was determined (R2= 0.92). These methods combined demonstrated that the glycine-site specific NMDA antagonist ACEA 1021 (4 × 30 mg/kg, i.p., 2 hourly) given 30 minutes before ( S)-MDMA (4 × 10 mg/kg, i.p., 2 hourly) was able to prevent the depletion of both 5-HT content and uptake site density but unable to prevent the depletion of 5-HT content and uptake site density caused dexfenfluramine (4 × 15 mg/kg, i.p., 2 hourly).
- Publication
Annals of the New York Academy of Sciences, 2000, Vol 914, Issue 1, p208
- ISSN
0077-8923
- Publication type
Article
- DOI
10.1111/j.1749-6632.2000.tb05197.x