We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Growth Inhibition and Apoptosis of Neuroblastoma Cells Through ROS-Independent MEK/ERK Activation by Sulforaphane.
- Authors
Hsu, Yi-Chiang; Chang, Sue-Joan; Wang, Miin-Yau; Chen, Yi-Ling; Huang, Tzuu-Yuan
- Abstract
Deregulation of apoptosis alters the balance of cell proliferation and cell death, resulting in a variety of diseases, including cancer. In recent studies, sulforaphane (SFN) has demonstrated potent anti-tumor and chemopreventive activities. A possible signal transduction pathway has also been elucidated for SFN-induced apoptosis in human neuroblastoma SH-SY5Y cells. The present study further investigates the anti-proliferation activities of SFN through induced apoptosis in SH-SY5Y cells. We found that treating SH-SY5Y cells with SFN resulted in the depletion of mitochondrial membrane potential (Δψ), which in turn increased caspase 9, caspase 3, and the up-regulation of phosphorylated MEK/ERK without generating reactive oxygen species. Results were confirmed by MTT assay, which demonstrated the cytotoxic activity of SFN against SH-SY5Y cells (IC values of 20 μM).
- Subjects
NEUROBLASTOMA; APOPTOSIS; CANCER; CELL proliferation; SULFORAPHANE
- Publication
Cell Biochemistry & Biophysics, 2013, Vol 66, Issue 3, p765
- ISSN
1085-9195
- Publication type
Article
- DOI
10.1007/s12013-013-9522-y