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- Title
Upregulation of MAP kinase HOG1 gene of white-rot fungus Phlebia sp. MG-60 inhibits the ethanol fermentation and mycelial growth.
- Authors
Taichi Motoda; Fu-Chia Chen; Taku Tsuyama; Yuji Tokumoto; Yoshio Kijidani; Ichiro Kamei
- Abstract
Wood biomass conversion for fossil resource replacement could result in the sustainable production of chemicals, although lignin represents an obstacle to efficient polysaccharide use. White-rot fungus Phlebia sp. MG-60 reportedly selectively and aerobically degrades lignin in hardwood, then it begins cellulose saccharification from the delignified wood to produce ethanol. Environmental conditions might change white-rot fungi-driven biomass conversion. However, how the environmental response sensor affects ethanol fermentation in white-rot fungi remains elusive. In this study, we focused on MGHOG1, the yeast Hog1 homolog in Phlebia sp. MG-60, a presumably important player in osmoresponse. We generated MGHOG1 overexpressing (OE) transformants in Phlebia sp. MG-60, exhibiting slower mycelial growth compared with the wild-type under salinity stress. MGHOG1 overexpressing liquid cultures displayed suppressed mycelial growth and ethanol fermentation. Therefore, MGHOG1 potentially influences ethanol fermentation and mycelial growth in Phlebia sp. MG-60. This study provides novel insights into the regulation of white-rot fungi-mediated biomass conversion.
- Subjects
MITOGEN-activated protein kinases; LIGNIN structure; ETHANOL; FERMENTATION; SUSTAINABILITY; LIGNINS; BIOMASS conversion; POLYSACCHARIDES
- Publication
Bioscience, Biotechnology & Biochemistry, 2023, Vol 87, Issue 2, p217
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1093/bbb/zbac203