We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Inhibitory effects of 3α-hydroxy-lup-20(29)-en-23, 28-dioic acid on lipopolysaccharide-induced TNF-α, IL-1β, and the high mobility group box 1 release in macrophages.
- Authors
Liu, Xiang-Qian; Zou, Qin-Peng; Huang, Jian-Jun; Yook, Chang-Soo; Whang, Wan-Kyunn; Lee, Hyeong-Kyu; Kwon, Ok-Kyoung
- Abstract
We investigated the anti-inflammatory effects of 3α-hydroxy-lup-20(29)-en-23, 28-dioic acid (HLEDA)—a lupane-type triterpene isolated from leaves of Acanthopanax gracilistylus W. W.Smith (AGS), as well as the underlying molecular mechanisms in lipopolysaccharide (LPS)-induced RAW264.7 cells. Our results demonstrated that HLEDA concentration-dependently reduced the production of nitric oxide (NO), significantly suppressed LPS-induced expression of TNF-α and IL-1β at the mRNA and protein levels in RAW264.7 cells. Further analysis revealed that HLEDA could reduce the secretion of High Mobility Group Box 1 (HMGB1). Additionally, the results showed that HLEDA efficiently decreased nuclear factor-kappaB (NF-κB) activation by inhibiting the degradation and phosphorylation of IκBα. These results suggest that HLEDA exerts anti-inflammatory properties in LPS-induced macrophages, possibly through inhibition of the NF-κB signaling pathway, which mediates the expression of pro-inflammatory cytokines. These results warrant further studies that would concern candidate therapy for diseases, such as fulminant hepatitis and rheumatology of triterpenoids in AGS.
- Subjects
ANTI-inflammatory agents; LIPOPOLYSACCHARIDES; TUMOR necrosis factors
- Publication
Bioscience, Biotechnology & Biochemistry, 2017, Vol 81, Issue 7, p1305
- ISSN
0916-8451
- Publication type
Article
- DOI
10.1080/09168451.2017.1301803