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- Title
Recombinant human estrogen, androgen and progesterone receptors for detection of potential endocrine disruptors.
- Authors
Scippo, Marie-Louise; Argiris, Catherine; Van De Weerdt, Cécile; Muller, Marc; Willemsen, Philippe; Martial, Joseph; Maghuin-Rogister, Guy
- Abstract
This work reports the binding capacity of various chemicals (so-called endocrine disruptors) to recombinant human steroid receptors (hERα, hPR and hAR). The tested chemicals are organochlorine insecticides (DDT and its metabolites, methoxychlor, aldrin, dieldrin, chlordecone, lindane, trichlorobenzene), estrogenic insecticides (endosulfan, toxaphene, nonachlor), herbicides (alachlor and atrazine), fungicides (benomyl and vinclozolin), industrial chemicals (nonylphenol, bisphenol A, diphenylphtalate), antioxidants (butylated hydroxyanisol) and some phytoestrogens. Except for phytoestrogens, most of the tested chemicals (DDT and its metabolites, aldrin, α- and β-endosulfan, toxaphen, trans-nonachlor) show higher affinities for hPR than for hERα, indicating that the interaction with the progesterone receptor could contribute to the endocrine-disrupting effects imputed to these chemicals. We propose to use binding assays using recombinant human steroid receptors as screening tools for the detection of endocrine disruptors in various samples.
- Subjects
STEROID hormones; ENDOCRINE diseases; PROGESTERONE receptors; ORGANOCHLORINE compounds; BIOLOGICAL products; CHEMICAL bonds
- Publication
Analytical & Bioanalytical Chemistry, 2004, Vol 378, Issue 3, p664
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-003-2251-0