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- Title
Electrochemical Magnetic Immunosensors for the Determination of Ceruloplasmin.
- Authors
Ojeda, Irene; Moreno‐Guzmán, María; González‐Cortés, Araceli; Yáñez‐Sedeño, Paloma; Pingarrón, José M.
- Abstract
Electrochemical immunosensors for ceruloplasmin (Cp) are reported for the first time. Two configurations involving magnetic beads (MBs) functionalized with Protein A or Streptavidin for immobilization of Cp antibodies were compared, using competitive immunoassay with synthesized alkaline phosphatase-Cp conjugate. Upon capturing MBs-immunoconjugates onto screen-printed carbon electrodes, quantification of Cp was accomplished by DPV measurement of 1-naphthol generated after 1-naphthylphosphate addition. Linear ranges of calibration curves and detection limits were 0.1-1000 µg/mL and 0.040 µg/mL (Protein A-MBs), and 0.025-20 µg/mL and 0.018 µg/mL (Strept-MBs). Good results were obtained in the determination of Cp in spiked human serum samples.
- Subjects
IMMUNOSENESCENCE; CERULOPLASMIN; STREPTAVIDIN; IMMUNOGLOBULINS; PHOSPHATASES; CARBON electrodes; ELECTROCHEMICAL apparatus
- Publication
Electroanalysis, 2013, Vol 25, Issue 9, p2166
- ISSN
1040-0397
- Publication type
Article
- DOI
10.1002/elan.201300269