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- Title
Synthesis of kyotorphin precursor from eutectic mixtures catalysed by α-chymotrypsin.
- Authors
Kim, Chulyoung; Lee, In Kyu; Ahn, Jung Eun; Shin, Chul Soo
- Abstract
The kyotorphin precursor, N-carbobenzoxyl-l-tyrosine-l-arginine amide (N-CBZ-l-Tyr-l-ArgNH2), was synthesized from N-CBZ-l-tyrosine ethyl ester (N-CBZ-l-TyrOEt) and l-arginine amide (l-ArgNH2) by using α-chymotrypsin. Eutectic mixtures were formed by mixing the substrates in the presence of water and/or organic solvents as adjuvants. The eutectic temperature was obtained with a 0.45 l-ArgNH2 mole fraction. It was lowered to 23 °C by addition of 10% (v/w) water and 5% (v/w) dimethyl formamide, thus maintaining homogeneous liquid states at the reaction temperature of 30 °C. After 9 h the solutions became solidified and no further reaction took place. Approximately 90% (mol/mol) conversion was achieved from the substrate mixtures with substrate contents responsible for more than 80% (w/w) of the total mixture.
- Subjects
CHYMOTRYPSIN; SERINE proteinases; TYROSINE; ORGANIC compounds; ARGININE; PLASTICIZERS
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 17, p1423
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1011678132456