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- Title
Optimization of conditions for the production of pullulan and high molecular weight pullulan by Aureobasidium pullulans.
- Authors
Lee, Ji-Hyun; Kim, Jeong-Hwa; Zhu, Il-Hui; Zhan, Xiao-Bei; Lee, Jin-Woo; Shin, Dong-Hoon; Kim, Sung-Koo
- Abstract
Aureobasidium pullulans had a maximum yield coefficient of pullulan (Yp/s=0.24) with an initial pH of the culture broth of 6.5 in a shake-flask culture. In a batch culture, the maximum pullulan yield coefficient of 0.30 was obtained at the aeration rate of 0.5 vvm. A yeast-like form and mycelial form of cells were found at the culture broth with pH controlled at 4.5 with a maximum yield coefficient of pullulan of 0.27. However, a high portion (35%) of high molecular weight pullulan (Mw>2 000 000) was produced at pH 6.5 with a yeast-like morphology of the cells.
- Subjects
GLUCOSIDASES; PULLULANASE; MOLECULAR weights; CULTURES (Biology); YEAST; MORPHOLOGY
- Publication
Biotechnology Letters, 2001, Vol 23, Issue 10, p817
- ISSN
0141-5492
- Publication type
Article
- DOI
10.1023/A:1010365706691