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- Title
基于UPLC-MS/MS定量检测法优化灵芝三萜热回流提取工艺的研究.
- Authors
彭小芳; 程池露; 唐庆九; 张劲松; 唐传红; 王晨光; 冯娜; 韩伟
- Abstract
Hot reflux extraction process was optimized for Ganoderma lingzhi triterpenoids based on ultra-high performance liquid-triple quadrupole mass spectrometry(UPLC-MS/MS) quantitative detection method. The established UPLC-MS/MS method was used to precisely detect the content of 19 triterpenoids of G. lingzhi fruiting bodies of seven cultivated varieties from different sources, and fruiting body of sd-2 G. lingzhi with high triterpenoids content was selected as the extractive material. Based on triterpenoid content and extract yield, the triterpenoid extraction process was optimized through single factor and response surface experiments. The best optimization conditions obtained were as follows: 75% of ethanol concentration, 2.5 h of extraction time, 14:1 of liquid-to-material ratio, and two times of repeated extraction. A pilot experiment was conducted for obtained optimal extraction technology, and the yield of the ethanol extract of G. lingzhi fruiting body was 6.10%, and the content of triterpenoid was 11.8591 mg/g. The results provide theoretical reference for the industrialized large-scale extraction of G. lingzhi triterpenoids and technical support of further development and utilization of G. lingzhi high value-added products.
- Subjects
FRUITING bodies (Fungi); TRITERPENOIDS; MASS spectrometry; PILOT projects; GANODERMA; ETHANOL
- Publication
Mycosystema, 2021, Vol 40, Issue 9, p2480
- ISSN
1672-6472
- Publication type
Article
- DOI
10.13346/j.mycosystema.210137