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- Title
糖尿病大鼠下丘脑Nesfatin-1 对胃运动的影响及机制研究.
- Authors
李峰; 王程; 柳洋; 高胜利
- Abstract
To observe the effect of Nesfatin-1 on the gastric motility of normal rat and diabetic rats and its potential mechanism. Normal rats were randomly divided into 0.08 滋g, 0.8 滋g, 8.0 滋g/0.5 滋L Nesfatin-1 group; 30 滋g/0.5 滋L astressin-B group; (0.8 滋g Nesfatin-1 + 30 滋gastressin-B) / 0.5 滋L group; (NR + SS) group; normal sheep serum + stimulated (NR + ES) group; anti-NUCB2 / Nesfatin-1 antibody + anti-Nn-Ab + SS) group; anti-NUCB2/Nesfatin-1 antibody + anti-Nn-Ab + ES group. The diabetic rats were randomly divided into 0.08 滋g/0.5 滋L Nesfatin-1 group, 0.8 滋g/0.5 滋L Nesfatin-1 group, 8.0 滋g/0.5 滋L Nesfatin-1 group, 0.5 滋L NS group, NR + SS NR + ES group; anti-Nn-Ab + SS group; anti-Nn-Ab + ES group. After the rat stomach was placed in the sensor, put a catheter into ventral medial nucleus, then observe the gastric motility of conscious rats and after electrical stimulation of hippocampal CA1 area. Compared with the normal saline group, Nesfatin-1 was injected into the ventral nucleus of the hypothalamus, and the amplitude and frequency of gastric contraction were significantly decreased. 0.5 滋L (0.8 滋g Nesfatin-1 + 30 滋gastressin-B) after mixed solution, the amplitude and frequency of gastric contraction were significantly increased in rats compared with 0.8 滋g Nesfatin-1 alone. Rats were injected with 0.5 滋L of Nesfatin-1 (0.8 滋g) in the ventral medial nucleus of the hypothalamus, and the amplitude and frequency of gastric contraction were significantly decreased in the ventral medial nucleus of the hypothalamus. 0.5 滋L (0.8 滋g Nesfatin-1 + 30 滋gastressin-B) after treatment, the amplitude and frequency of gastric contraction were significantly higher in rats than 0.8 滋g Nesfatin-1 alone. In the hypothalamic nucleus of the hypothalamus, the anti-NUCB2/Nesfatin-1 antibody was used to stimulate the hippocampal CA1 region, and the amplitude and frequency of gastric contraction were further enhanced compared with the normal sheep serum +/Nesfatin-1 antibody was used to stimulate the hippocampal CA1 region. Compared with the anti-NUCB2/Nesfatin-1 antibody + pseudo-electrical stimulation group, the amplitude and frequency of gastric contraction were significantly increased. The NAGB2/Nesfatin-1 antibody was injected into the ventral medial nucleus of the hypothalamus to induce the electrical stimulation of the hippocampal CA1 region. Compared with the normal sheep serum + electric stimulation group, the gastric motility index of the normal rats and diabetic rats were significantly increased, The gastric motility index of normal and diabetic rats was significantly higher than that of anti-NUCB2/Nesfatin-1 antibody + pseudo-electrical stimulation group after injection of anti-NUCB2/Nesfatin-1 antibody in the hippocampal CA1 region. Compared with normal rats, the rats were injected with anti-NUCB2/Nesfatin-1 antibody in the hippocampal CA1 region and the hypothalamic ventral nucleus. The rats were injected with anti-NUCB2 / Nesfatin-1 antibody. There was no significant difference in gastric motility index between diabetic rats. Hippocampus-hypothalamic Nesfatin-1 signaling pathway is involved in gastric afferent information and gastric motility regulation, which may be related to CRF systemactivity.
- Publication
Progress in Modern Biomedicine, 2018, Vol 18, Issue 6, p1060
- ISSN
1673-6273
- Publication type
Article
- DOI
10.13241/j.cnki.pmb.2018.06.012