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- Title
Direct induction of ramified microglia-like cells from human monocytes: Dynamic microglial dysfunction in Nasu-Hakola disease.
- Authors
Masahiro Ohgidani; Kato, Takahiro A.; Daiki Setoyama; Noriaki Sagata; Ryota Hashimoto; Kazue Shigenobu; Tetsuhiko Yoshida; Kohei Hayakawa; Norihiro Shimokawa; Daisuke Miura; Hideo Utsumi; Shigenobu Kanba
- Abstract
Microglia have been implicated in various neurological and psychiatric disorders in rodent and human postmortem studies. However, the dynamic actions of microglia in the living human brain have not been clarified due to a lack of studies dealing with in situmicroglia. Herein, we present a novel technique for developing induced microglia-like (iMG) cells from human peripheral blood cells. An optimized cocktail of cytokines, GM-CSF and IL-34, converted human monocytes into iMG cells within 14 days. The iMG cells have microglial characterizations; expressing markers, forming a ramified morphology, and phagocytic activity with various cytokine releases. To confirm clinical utilities, we developed iMG cells from a patient of Nasu-Hakola disease (NHD), which is suggested to be directly caused by microglial dysfunction, and observed that these cells from NHD express delayed but stronger inflammatory responses compared with those from the healthy control. Altogether, the iMG-technique promises to elucidate unresolved aspects of human microglia in various brain disorders.
- Subjects
MICROGLIA; MONOCYTES; BLOOD cells; CYTOKINES; NEUROLOGICAL research; BRAIN disease research
- Publication
Scientific Reports, 2014, p1
- ISSN
2045-2322
- Publication type
Article
- DOI
10.1038/srep04957