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- Title
Single-Cell RNA-Sequencing Reveals Heterogeneity and Transcriptional Dynamics in Porcine Circulating CD8 + T Cells.
- Authors
Han, Pingping; Guo, Yaping; Zhang, Wei; Wang, Daoyuan; Wu, Yalan; Li, Xinyun; Zhu, Mengjin
- Abstract
Pigs are the most important source of meat and valuable biomedical models. However, the porcine immune system, especially the heterogeneity of CD8 T cell subtypes, has not been fully characterized. Here, using single-cell RNA sequencing, we identified 14 major cell types from peripheral blood circulating cells of pigs and observed remarkable heterogeneity among CD8 T cell types. Upon re-clustering of CD8+ T cells, we defined four CD8 T cell subtypes and revealed their potential differentiation trajectories and transcriptomic differences among them. Additionally, we identified transcription factors with potential regulatory roles in maintaining CD8 T cell differentiation. The cell-cell communication analysis inferred an extensive interaction between CD8 T cells and other immune cells. Finally, cross-species analysis further identified species-specific and conserved cell types across different species. Overall, our study provides the first insight into the extensive functional heterogeneity and state transitions among porcine CD8 T cell subtypes in pig peripheral blood, complements the knowledge of porcine immunity, and enhances its potential as a biomedical model.
- Subjects
CD8 antigen; T cell differentiation; RNA sequencing; T cells; HETEROGENEITY; BLOOD cells; PORCINE reproductive &; respiratory syndrome
- Publication
Cells (2073-4409), 2024, Vol 13, Issue 8, p692
- ISSN
2073-4409
- Publication type
Article
- DOI
10.3390/cells13080692