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- Title
The Garlic Compound, Diallyl Trisulfide, Attenuates Benzo[a]Pyrene-Induced Precancerous Effect through Its Antioxidant Effect, AhR Inhibition, and Increased DNA Repair in Human Breast Epithelial Cells.
- Authors
Ferguson, Dominique T.; Taka, Equar; Messeha, Samia; Flores-Rozas, Hernan; Reed, Sarah L.; Redmond, Bryan V.; Soliman, Karam F. A.; Kanga, Konan J. W.; Darling-Reed, Selina F.
- Abstract
Exposure to B[a]P, the most characterized polycyclic aromatic hydrocarbon, significantly increases breast cancer risk. Our lab has previously reported that diallyl trisulfide (DATS), a garlic organosulfur compound (OSC) with chemopreventive and cell cycle arrest properties, reduces lipid peroxides and DNA damage in normal breast epithelial (MCF-10A) cells. In this study, we evaluated the ability of DATS to block the B[a]P-induced initiation of carcinogenesis in MCF-10A cells by examining changes in proliferation, clonogenic formation, reactive oxygen species (ROS) formation, 8-hydroxy-2-deoxyguanosine (8-OHdG) levels, and protein expression of ARNT/HIF-1β, CYP1A1, and DNA POLβ. The study results indicate that B[a]P increased proliferation, clonogenic formation, ROS formation, and 8-OHdG levels, as well as increasing the protein expression of ARNT/HIF-1β and CYP1A1 compared to the control. Conversely, DATS/B[a]P co-treatment (CoTx) inhibited cell proliferation, clonogenic formation, ROS formation, and 8-OHdG levels compared to B[a]P alone. Treatment with DATS significantly inhibited (p < 0.0001) AhR expression, implicated in the development and progression of breast cancer. The CoTx also attenuated all the above-mentioned B[a]P-induced changes in protein expression. At the same time, it increased DNA POLβ protein expression, which indicates increased DNA repair, thus causing a chemopreventive effect. These results provide evidence for the chemopreventive effects of DATS in breast cancer prevention.
- Subjects
BREAST tumor prevention; ALKENES; CARCINOGENESIS; SULFIDES; ANTIOXIDANTS; CELL receptors; HYDROCARBONS; CELL cycle; GENE expression; GARLIC; CELL proliferation; RESEARCH funding; PLANT extracts; DNA repair; CELL lines; EPITHELIAL cells; REACTIVE oxygen species; PRECANCEROUS conditions; CHEMOPREVENTION; BREAST tumors
- Publication
Nutrients, 2024, Vol 16, Issue 2, p300
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu16020300