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- Title
Microbiota-Dependent Upregulation of Bitter Taste Receptor Subtypes in the Mouse Large Intestine in High-Fat Diet-Induced Obesity.
- Authors
Caremoli, Filippo; Huynh, Jennifer; Lagishetty, Venu; Markovic, Daniela; Braun, Jonathan; Dong, Tien S.; Jacobs, Jonathan P.; Sternini, Catia
- Abstract
Bitter taste receptors (Tas2rs in mice) detect bitterness, a warning signal for toxins and poisons, and are expressed in enteroendocrine cells. We tested the hypothesis that Tas2r138 and Tas2r116 mRNAs are modulated by microbiota alterations induced by a long-term high-fat diet (HFD) and antibiotics (ABX) (ampicillin and neomycin) administered in drinking water. Cecum and colon specimens and luminal contents were collected from C57BL/6 female and male mice for qRT-PCR and microbial luminal 16S sequencing. HFD with/without ABX significantly increased body weight and fat mass at 4, 6, and 8 weeks. Tas2r138 and Tas2r116 mRNAs were significantly increased in mice fed HFD for 8 weeks vs. normal diet, and this increase was prevented by ABX. There was a distinct microbiota separation in each experimental group and significant changes in the composition and diversity of microbiome in mice fed a HFD with/without ABX. Tas2r mRNA expression in HFD was associated with several genera, particularly with Akkermansia, a Gram-negative mucus-resident bacterium. These studies indicate that luminal bacterial composition is affected by sex, diet, and ABX and support a microbial dependent upregulation of Tas2rs in HFD-induced obesity, suggesting an adaptive host response to specific diet-induced dysbiosis.
- Subjects
LARGE intestine physiology; OBESITY; REVERSE transcriptase polymerase chain reaction; SEQUENCE analysis; BODY weight; GUT microbiome; ANIMAL experimentation; GRAM-negative bacteria; CELL receptors; MESSENGER RNA; RESEARCH funding; TASTE; MICE; ADIPOSE tissues; ANTIBIOTICS
- Publication
Nutrients, 2023, Vol 15, Issue 19, p4145
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu15194145