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- Title
Sodium crocetinate does not alter gut hypercapnic responses or renal energy stores during transient sub-diaphragmatic ischaemia.
- Authors
Morgan, Thomas J.; Venkatesh, Balasubramanian; Crerar-Gilbert, Agnieszka; Willgoss, Desley; Endre, Zoltan H.
- Abstract
<bold>Objectives: </bold>To evaluate the protection afforded by trans-sodium crocetinate against dysoxia in an animal model of recurrent sub-diaphragmatic ischaemia.<bold>Design: </bold>Prospective experimental animal study.<bold>Setting: </bold>University research laboratory<bold>Subjects: </bold>Adult male Sprague-Dawley rats.<bold>Interventions: </bold>Twelve adult male Sprague-Dawley rats (340-510 g) were anaesthetised with sodium pentobarbitone 60 mg/kg i.p. and ventilated with oxygen and isoflurane via tracheostomy. Six 2-min episodes of sub-diaphragmatic hypotension (mean pressure 30 mmHg) were induced using a sling around the proximal aorta. Before the third and sixth episodes, saline 1.5 ml/kg was injected into the aortic cannula. In six rats, this saline contained trans-sodium crocetinate 50 microg/ml.<bold>Measurements and Main Results: </bold>Ileal luminal PCO(2) and distal aortic pressure were monitored continuously. Following ischaemic episodes trans-sodium crocetinate had no discernible effect on either degree of PCO(2) elevation or the time to peak PCO(2). No effects on renal energy charge or nucleotide concentrations were detected. UV-visible spectroscopy of the crocetinate preparation showed that some cis isomer was present.<bold>Conclusions: </bold>The findings, although limited to one drug dosage in one animal model, bring into question whether trans-sodium crocetinate affects plasma oxygen diffusivity in vivo. Alternative explanations for the negative findings include a TSC-induced exacerbation of arterio-venous oxygen shunting, the brevity of the dysoxic episodes, and the presence of cis isomer.
- Subjects
ISCHEMIA; LABORATORY animals; ANESTHETICS; ARTERIES; BLOOD circulation; CHEST (Anatomy); ANIMAL experimentation; BIOLOGICAL models; CARBON dioxide; CAROTENOIDS; HYPERCAPNIA; INTESTINAL mucosa; KIDNEYS; LONGITUDINAL method; RATS
- Publication
Intensive Care Medicine, 2003, Vol 29, Issue 4, p652
- ISSN
0342-4642
- Publication type
journal article
- DOI
10.1007/s00134-003-1641-2