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- Title
Production of Extracellular Matrix Proteins by Human Pulp Fibroblasts in Contact with Papacárie and Carisolv.
- Authors
Bussadori, Sandra Kalil; Silverio Amancio, Olga Maria; Martins, Manoela Domingues; Cardoso Guedes, Carolina; Alfaya, Thays Almeida; Santos, Elaine Marcílio; Miranda França, Cristiane
- Abstract
Purpose: To evaluate the effect of two products for the chemomecanical removal of carious tissue (Papacárie and Carisolv) on human dental pulp fibroblasts and the synthesis of extracellular matrix proteins. Materials and Methods: Fibroblasts were divided into three groups: group 1 (control), group 2 (Papacárie) and group 3 (Carisolv). Collagen I, III, fibronectin and osteonectin were analysed by immunofluorescence and compared among the groups. Results: The groups exhibited similar immunolabeling for vimentin, type I collagen and fibronectin, but were negative for type III collagen. Osteonectin staining was strongly positive in the cells treated with Papacárie and Carisolv and weakly positive in the control group. Conclusion: The findings of the present study showed that Papacárie and Carisolv are not cytotoxic to pulp fibroblast cells. Moreover, these products stimulate fibroblasts to produce osteonectin, likely leading to the formation of dentin matrix. These findings confirm the safe, beneficial use of both gels in minimally invasive techniques.
- Subjects
EXTRACELLULAR matrix proteins; DENTAL pulp; TREATMENT of dental caries; OSTEONECTIN; IMMUNOFLUORESCENCE
- Publication
Oral Health & Preventive Dentistry, 2014, Vol 12, Issue 1, p55
- ISSN
1602-1622
- Publication type
Article
- DOI
10.3290/j.ohpd.a31225