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- Title
αB-Crystallin regulates expansion of CD11b<sup>+</sup>Gr-1<sup>+</sup> immature myeloid cells during tumor progression.
- Authors
Dieterich, Lothar C.; Schiller, Petter; Hua Huang; Wawrousek, Eric F.; Loskog, Angelica; Wanders, Alkwin; Moons, Lieve; Dimberg, Anna
- Abstract
The molecular chaperone αB-crystallin has emerged as a target for cancer therapy due to its expression in human tumors and its role in regulating tumor angiogenesis, αB-crystallin also reduces neuroinflammation, but its role in other inflammatory conditions has not been investigated. Here, we examined whether αB-crystallin regulates inflammation associated with tumors and ischemia. We found that CD45+ leukocyte infiltration is 3-fold increased in tumors and ischemic myocardium in αB-crystallin-deficient mice. Notably, αB-crystallin is prominently expressed in CD11b+ Gr-1+ immature myeloid cells (IMCs), known as regulators of angiogenesis and immune responses, while lymphocytes and mature granulocytes show low αB-crystallin expression, αB-Crystallin deficiency results in a 3-fold higher accumulation of CD11b+ Gr-1+ IMCs in tumors and a significant rise in CD11b+ Gr-1+ IMCs in spleen and bone marrow. Similarly, we noted a 2-fold increase in CD11b+ Gr-1+ IMCs in chronically inflamed livers in αB-crystallin-deficient mice. The effect of αB-crystallin on IMC accumulation is limited to pathological conditions, as CD11b+ Gr-1+ IMCs are not elevated in naive mice. Through ex vivo differentiation of CD11b+ Gr-1+ cells, we provide evidence that αB-crystallin regulates systemic expansion of IMCs through a cell-intrinsic mechanism. Our study suggests a key role of αB-crystaUin in limiting expansion of CD11bα Gr-1α IMCs in diverse pathological conditions.
- Subjects
CRYSTALLINS; CANCER treatment; NEOVASCULARIZATION; HEAT shock proteins; INFLAMMATION
- Publication
FASEB Journal, 2013, Vol 27, Issue 1, p151
- ISSN
0892-6638
- Publication type
Article
- DOI
10.1096/fj.12-213017