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- Title
Profiling and Identification of the Metabolites of Evodiamine in Rats using Ultra-Performance Liquid Chromatography with Linear Ion Trap Orbitrap Mass Spectrometer.
- Authors
Fang Ping Wu; Yong Yang; Liang Hong Liu; Hong Pu; Ping Jin; Zu Fu Yao; Wei Cai
- Abstract
Purpose: To develop a highly sensitive and specific ultra-performance liquid chromatography with linear ion trap Orbitrap mass spectrometer (UPLC-LTQ-Orbitrap) method to profile and identify the metabolites of evodiamine in rats. Methods: First, blood samples were collected after oral administration of evodiamine to rats (50 mg/kg). Next, the plasma samples were pretreated using a solid-phase extraction (SPE) method. Finally, all the samples were analyzed by ultra-performance liquid chromatography LTQ-Orbitrap mass spectrometry (UPLC-LTQ-Orbitrap) coupled with electrospray ionization source (ESI) in negative mode. Results: A total of 7 metabolites (2 phase I and 4 phase II metabolites, including 4 new metabolites, viz, 10-hydroxyevodiamine sulfate, 10-hydroxyevodiamine sulfate, 10-hydroxyevodiamine glucuronide and 3-hydroxyevodiamine glucuronide) as well as the parent drug itself, were detected and identified based on accurate mass measurements, fragmentation patterns, and chromatographic retention times. The in vivo metabolic reactions of evodiamine in rats were hydroxylation, hydroxylation + sulfate conjugation, and hydroxylation + glucuronidation. Conclusion: These results provide better understanding of the metabolism of evodiamine as well as strong indications of the effective forms of the drug in vivo.
- Subjects
QUINAZOLINE; LIQUID chromatography; ELECTROSPRAY ionization mass spectrometry; GLUCURONIDATION; HYDROXYLATION; SOLID phase extraction; CHINESE medicine
- Publication
Tropical Journal of Pharmaceutical Research, 2016, Vol 115, Issue 3, p623
- ISSN
1596-5996
- Publication type
Article
- DOI
10.4314/tjpr.v15i3.26