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- Title
Effect of Leptin on Insulin Resistance of Muscle -- Direct or Indirect?
- Authors
Doh, K. O.; Park, J. O.; Kim, Y. W.; Park, S. Y.; Jeong, J. H.; Jeon, J. R.; Lee, S. K.; Kim, J. Y.
- Abstract
We examined the effect of leptin on the insulin resistance in skeletal muscles by measuring glucose transport. Male Wistar rats were fed rat chow or high-fat diets for 30 days. Before sacrifice, rats fed high-fat diet were subcutaneously injected with leptin (1 mg/kg b.w.) for 3 days. The glucose transport in epitrochlearis and soleus muscles did not differ in the experimental groups under basal conditions, however these values decreased significantly in the rats fed high-fat diet under insulin stimulation (p<0.01). Leptin treatment recovered the decreased glucose transport in epitrochlearis (p<0.05) and soleus muscles (p=0.08). Triglyceride concentrations in soleus muscles were increased significantly in the rats fed high-fat diet as compared to rats fed chow diet (p<0.01), and were decreased significantly by leptin treatment (p<0.01). The glucose transport was measured under basal conditions and after 60 µU/ml of insulin treatment with or without 50 ng/ml of leptin. Leptin had no direct stimulatory effect on glucose transport under both basal and insulinstimulated conditions in vitro. These results demonstrate that leptin injection to rats fed high-fat diet recovered impaired insulin responsiveness of skeletal muscles and muscle triglyceride concentrations. However, there was no direct stimulatory effect of leptin on insulin sensitivity of skeletal muscles in vitro.
- Subjects
LEPTIN; INSULIN; GLUCOSE; MUSCULOSKELETAL system; TRIGLYCERIDES
- Publication
Physiological Research, 2006, Vol 55, Issue 4, p413
- ISSN
0862-8408
- Publication type
Article
- DOI
10.33549/physiolres.930799