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- Title
Oxidative stress and antioxidant status in type 1 diabetes mellitus.
- Authors
VESSBY, J.; BASU, S.; MOHSEN, R.; BERNE, C.; VESSBY, B.
- Abstract
<bold>Objectives: </bold>To test the hypothesis that type 1 diabetes is associated with increased oxidative stress and/or antioxidant status by investigating concentrations of 8-iso-prostaglandin F2alpha (8-iso-PGF2alpha) in urine and plasma and malondialdehyde (MDA) in plasma as indicators of lipid peroxidation in vivo, and antioxidant status in diabetic subjects compared with healthy control subjects.<bold>Design and Subjects: </bold>Thirty-eight subjects with type 1 diabetes mellitus and 41 healthy age- and sex-matched control subjects were included in the study. Blood and urine samples were obtained and analysed for 8-iso-PGF2alpha with a newly developed radioimmunoassay, as well as for MDA, total antioxidant capacity (TAOC) and serum tocopherol levels.<bold>Results: </bold>None of the variables of lipid peroxidation showed any significant difference between the two groups. Similarly, there were no significant correlations between the levels of 8-iso-PGF2alpha or MDA, and degree of glycemic control (HbA1c). Total antioxidant capacity in plasma was 16% lower amongst the subjects with type 1 diabetes than in the control group (P < 0.0005). Lipid corrected levels of alpha-tocopherol in serum were significantly increased in type 1 diabetic subjects (P < 0.05), as were gamma-tocopherol levels (P < 0.005).<bold>Conclusions: </bold>In spite of lower total antioxidant defence, our results do not support the oxidative stress hypothesis for type 1 diabetes mellitus. The higher tocopherol levels suggest that no vitamin E supplementation is necessary for subjects with type 1 diabetes mellitus.
- Subjects
DIABETES; ANTIOXIDANTS; PEROXIDATION; MALONDIALDEHYDE; ARACHIDONIC acid; COMPARATIVE studies; HIGH performance liquid chromatography; TYPE 1 diabetes; RESEARCH methodology; MEDICAL cooperation; LIPID peroxidation (Biology); PROSTAGLANDINS; RADIOIMMUNOASSAY; RESEARCH; VITAMIN E; OXIDATIVE stress; EVALUATION research; CASE-control method
- Publication
Journal of Internal Medicine, 2002, Vol 251, Issue 1, p69
- ISSN
0954-6820
- Publication type
journal article
- DOI
10.1046/j.1365-2796.2002.00927.x