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- Title
Modulation of Charge Density of Cationic Conjugated Polyelectrolytes for Improving the FRET-Induced Sensory Signal with Enhanced On/Off Ratio.
- Authors
Jeong, Ji‐Eun; Jung, In Hwan; Kim, Boram; Le, Van Sang; Woo, Han Young
- Abstract
Three types of cationic polyfluorenes with a different number of ionic groups are synthesized to modulate their solubility in water and the interactions with anionic oligonucleotide probes (DNA-C). Increasing the charge density improves the water-solubility of conjugated polyelectrolytes (CPEs) but decreases its optical antenna effect in the electrostatic sensory platform (CPE/DNA-C), resulting in low fluorescence resonance energy transfer (FRET) signal. With increasing charge density in CPE, the concentration of conjugated units in CPE/DNA-C decreases at [+]:[−] = 1:1, after which little driving force is expected to bring positive CPEs and negative DNA-C into close proximity for efficient FRET. CPF2 with 2 ionic groups (per repeat unit) and hydrophilic polyether side-chains exhibits good water-solubility and a higher FRET signal with an enhanced signal on/off ratio. In addition, the charge density in CPEs modulates the range of sensory responses by forming a tighter or weaker electrostatic CPE/DNA-C complex, which perturbs the probe-target binding interactions.
- Subjects
CHARGE density waves; POLYELECTROLYTES; POLYFLUORENES
- Publication
Macromolecular Chemistry & Physics, 2016, Vol 217, Issue 3, p459
- ISSN
1022-1352
- Publication type
Article
- DOI
10.1002/macp.201500304