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- Title
Growth and respiration of rice ( Oryza sativa L.) Cells in suspension culture.
- Authors
Brown, Dennis; Beevers, Harry
- Abstract
Studies on the growth and respiration of batch suspension cultures of rice ( Oryza sativa L.) in a reference medium containing Murashige-Skoog salts, 2% (w/v) sucrose and yeast extract are reported. It was found that the yeast extract contributed 70% of the phosphate in this medium, and that the cells grew equally well in continued subculture in a defined medium which contained 6 mM phosphate and 3% (w/v) sucrose and the remaining Murashige-Skoog salts. Cell clumps (up to 1.5 mm diameter) were prevalent in the initial cultures in the reference medium. In such cultures the critical O pressure of cell respiration was high (∼125 μM), and ethanol accumulated. When cell clumps were routinely removed during several weekly subcultures on the defined medium cultures were obtained in which no clumps were present, the critical O pressures was decreased to 40 μM and no ethanol accumulated.
- Publication
Plant Cell, Tissue & Organ Culture, 1987, Vol 10, Issue 3, p175
- ISSN
0167-6857
- Publication type
Article
- DOI
10.1007/BF00037302