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- Title
Simultaneous Qualitative and Quantitative Evaluation of the Coptidis Rhizoma and Euodiae Fructus Herbal Pair by Using UHPLC-ESI-QTOF-MS and UHPLC-DAD.
- Authors
Li, Yan-Ying; Guo, Min-Qun; Li, Xue-Mei; Yang, Xiu-Wei; Yoon, In-Soo; Cho, Hyun-Jong
- Abstract
The herbal pair of Coptidis Rhizoma (CR) and Euodiae Fructus (EF) is a classical traditional Chinese medicine formula used for treating gastro-intestinal disorders. In this study, we established a systematic method for chemical profiling and quantification analysis of the major constituents in the CR-EF herbal pair. A method of ultra high performance liquid chromatography/quadrupole time-of-flight mass spectrometry (UHPLC-QTOF-MS) for qualitative analysis was developed. Sixty-five compounds, including alkaloids, phenolics, and limonoids, were identified or tentatively assigned by comparison with reference standards or literature data. The UHPLC fingerprints of 19 batches of the CR-EF herbal pair samples were obtained and the reference fingerprint chromatograms were established. Furthermore, nine compounds among 24 common peaks of fingerprints were considered as marker components, which either had high contents or significant bioactivities, were applied to quality control of the CR-EF herbal pair by quantitative analysis. This UHPLC-DAD analysis method was validated by precision, linearity, repeatability, stability, recovery, and so on. The method was simple and sensitive, and thus reliable for quantitative and chemical fingerprint analysis for the quality evaluation and control of the CR-EF herbal pair and related traditional Chinese medicines.
- Subjects
TIME-of-flight mass spectrometry; QUADRUPOLE ion trap mass spectrometry; HIGH performance liquid chromatography; QUANTITATIVE chemical analysis; CHINESE medicine; CHEMICAL fingerprinting
- Publication
Molecules, 2020, Vol 25, Issue 20, p4782
- ISSN
1420-3049
- Publication type
Article
- DOI
10.3390/molecules25204782