We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
鳗鲡疱疹病毒实时荧光重组酶辅助扩增(RAA) 检测方法的建立及应用.
- Authors
陈曦; 杨金先; 葛均青
- Abstract
In order to establish a real-time fluorescence recombinase-aided amplification (RAA) method for detection of Anguillid herpesvirus (AngHV), a primer pair and probe targeting ORF95 sequence of AngHV was designed, followed by optimizing the reaction temperature and fixing the reaction time. The results showed that the established real-time fluorescence RAA had optimal reaction temperature of 39 ℃, with the reaction time of 20 min. The further evaluation of sensitivity, specificity, and repeatability showed that the detection method had the minimum AngHV detection concentration of 1×10 2 copies/ μL and had high specificity on AngHV, and showed no cross amplification reaction with American eel adomavirus ( AEAdoV), Rana grylio virus ( RGV), Koi herpesvirus (KHV), and White spot syndrome virus (WSSV). There was less than 5% of co-efficiency of variation within and between groups for the method which was then applied to analyse 25 collected samples. It was found that the detection rate of the real-time fluorescence RAA was consistent with that by qPCR of 92%, but much higer than PCR with the detection rate of 76%. These results revealed that the established RAA method for detection of AngHV was rapid, sensitive, reliable, and accurate, which is used for rapid clinical detection and conducting epidemiological investigations on AngHV.
- Publication
Journal of Dalian Ocean University, 2024, Vol 39, Issue 2, p234
- ISSN
2095-1388
- Publication type
Article
- DOI
10.16535/j.cnki.dlhyxb.2023-216