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- Title
DIAGNOSTIC VALUE OF PALUTOP®+4 OPTIMA ANTIGEN DETECTION TO MICROSCOPY GOLD STANDARD AND POLYMERASE CHAIN REACTION.
- Authors
Butarbutar, Trieva Verawaty; Milka, Amerensi; Adiatmaja, Christophorus Oetama; Aryati; Wardhani, Puspa
- Abstract
Palutop®+4 Optima is the newest rapid diagnostic test (RDT) to identify malaria with 4-line tests by detecting three malaria proteins, which are HRP-2, Pv-LDH and pLDH. Recently, microscopy provides the key standard among all the available malaria tests. However, it has many drawbacks while RDT with 4-line tests is still rare. This investigation would like to look for the diagnostic value from the Palutop®+4 Optima in comparison with microscopy and RT PCR as a gold standard and to utilise it in determining the cut-off parasitemia index. The samples, 105 whole blood samples were generated from the Merauke General Hospital, in Papua during November 2018 - June 2019 having fulfilled both inclusion and exclusion criteria. The examination of the samples was performed by thick and thin dropping which then analysed by employing the test kits, which were RT PCR and Palutop®+4 Optima. Compared to microscopy, Palutop®+4 Optima displayed Sn 100%, Sp 98.04%, PPV 98.18%, NPV 100%, LR+=51 and LR-=0, with the diagnostic accuracy at 99.05%. Meanwhile, in comparison with RT PCR, it presented a Sn 75.34%, Sp 100%, PPV 100%, NPV 64%, LR+= - and LR-=0.25 with diagnostic accuracy at 82.86%. The cut-off of parasitemia index in Palutop® + 4 Optima based on the ROC curve could not be determined. A very high sensitivy was exhibited by Palutop®+4 Optima's diagnostic value along with a high specificity in indicating the antigens of malaria, P. vivax, P. falciparum mixed Plasmodium infection.
- Subjects
NEW Guinea (Island); POLYMERASE chain reaction; MICROSCOPY; RECEIVER operating characteristic curves; ANTIGENS; MALARIA; PARASITEMIA
- Publication
Biochemical & Cellular Archives, 2022, Vol 22, Issue 1, p1721
- ISSN
0972-5075
- Publication type
Article