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- Title
Genomic characterization of thermophilicGeobacillusspecies isolated from the deepest sea mud of the Mariana Trench.
- Authors
Takami, Hideto; Nishi, Shinro; Lu, Jei; Shimamura, Shigeru; Takaki, Yoshihiro
- Abstract
The thermophilic strains HTA426 and HTA462 isolated from the Mariana Trench were identified asGeobacillus kaustophilusandG. stearothermophilus, respectively, based on physiologic and phylogenetic analyses using 16S rDNA sequences and DNA-DNA relatedness. The genome size of HTA426 and HTA462 was estimated at 3.23-3.49 Mb and 3.7-4.49 Mb, respectively. The nucleotide sequences of three independent ?-phage inserts ofG. stearothermophilusHTA462 have been determined. The organization of protein coding sequences (CDSs) in the two ?-phage inserts was found to differ from that in the contigs corresponding to each ? insert assembled by the shotgun clones of theG. kaustophilusHTA426 genome, although the CDS organization in another ? insert is identical to that in the HTA426 genome.
- Subjects
THERMOPHILIC bacteria; THERMOPHILIC microorganisms; NUCLEOTIDE sequence; GRAM-positive bacteria; BACTERIOLOGY; MICROBIOLOGY
- Publication
Extremophiles, 2004, Vol 8, Issue 5, p351
- ISSN
1431-0651
- Publication type
Article
- DOI
10.1007/s00792-004-0394-3