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- Title
Reusing Umbilical Cord Tissue Sample for Explant Extraction and Isolation of Wharton's Jelly Mesenchymal Stem Cells: A Qualitative Study.
- Authors
Noor Azlan, Noor Anastasha Balqis; Vitus, Vieralynda; Ahmad Shamsuddin, Shamsul Azlin B.; Razali, Nuguelis; Rashid, Nurshamimi Nor; Kamarul Zaman, Wan Safwani Wan
- Abstract
Introduction: Wharton's Jelly derived mesenchymal stem cells (WJMSC) have been a preferred source for stem cell studies for their non-invasive and ethical source and high proliferation ability. Several methods have been developed to isolate WJMSC, and currently, the main method that has been used is explant culture and enzymatic dissociation method. However, the tissues cannot be used after the enzymatic dissociation and to date, the tissues are discarded after single explant culture. Thus, this study would explore the possibility of reusing the tissue from the first explant culture into several explant cultures to optimise a single tissue sample usage. Methods: Wharton's Jelly was pinched and cut off from a cut open umbilical cord section into about 2 mm to 5 mm size and seeded into a T25 flask. After 3 days of culture, the tissue fragments were removed, washed and seeded onto a new flask. This step was repeated 5 more times. The morphology of the cells was observed under a microscope, and photos of them were taken. Results: From the microscopic observation taken after 6 days, we found that the tissue fragments were still viable for seeding even after reseeding 5 times with little to no contamination or losing senescence. This method would help maximise the number of cells isolated and expanded from one tissue sample. Further studies on this method should be done to validate the characteristics of the WJMSC expanded mesenchymal stem cells.
- Subjects
MESENCHYMAL stem cells; UMBILICAL cord; JELLY; CELL morphology; SEED size
- Publication
Malaysian Journal of Medicine & Health Sciences, 2022, Vol 18, p28
- ISSN
1675-8544
- Publication type
Article