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- Title
Expression and enzyme activity of cytochrome P450 enzymes CYP3A4 and CYP3A5 in human skin and tissue‐engineered skin equivalents.
- Authors
Smith, Sarah A.; Colley, Helen E.; Sharma, Parveen; Slowik, Klaudia M.; Sison‐young, Rowena; Sneddon, Andrew; Webb, Steven D.; Murdoch, Craig
- Abstract
Abstract: CYP3A4 and CYP4A5 share specificity for a wide range of xenobiotics with the CYP3 subfamily collectively involved in the biotransformation of approximately 30% of all drugs. CYP3A4/5 mRNA transcripts have been reported in the skin, yet knowledge of their protein expression and function is lacking. In this study, we observed gene and protein expression of CYP3A4/5 in both human skin and tissue‐engineered skin equivalents (TESEs), and enzyme activity was detected using the model substrate benzyl‐O‐methyl‐cyanocoumarin. Mass spectrometric analysis of TESE lysates following testosterone application revealed a time‐dependent increase in metabolite production, confirming the functional expression of these enzymes in skin.
- Subjects
XENOBIOTIC biodegradation; SKIN tests; CYTOCHROME P-450 regulation; MASS spectrometry; BIOTRANSFORMATION (Metabolism)
- Publication
Experimental Dermatology, 2018, Vol 27, Issue 5, p473
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/exd.13483