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- Title
Generation of Helios reporter mice and an evaluation of the suppressive capacity of Helios<sup>+</sup> regulatory T cells in vitro.
- Authors
Sugita, Kazunari; Hanakawa, Sho; Honda, Tetsuya; Kondoh, Gen; Miyachi, Yoshiki; Kabashima, Kenji; Nomura, Takashi
- Abstract
Helios is a member of the Ikaros transcription factor family and has been reported to be a marker of thymus-derived regulatory T cells (Treg). Helios is an intracellular protein, however, and hence cannot be used as a marker to separate living Tregs. To solve this problem, we generated Helios reporter mice in which Helios+ cells selectively express Venus, a variant of green fluorescent protein. Most of the Tregs in the thymus expressed Helios, whereas its expression was varied in peripheral lymphoid organs. The Helios+ Treg-population was superior in ability to suppress both antigen-specific and TCR-stimulated T cell responses. We also showed that Helios+ Tregs inhibited the cytokine production by T cells more efficiently than Helios- Tregs. We conclude that Helios reporter mouse strain is a useful tool to study function of Helios and that Helios+ Tregs represent the highly suppressive population.
- Subjects
SILENE (Genus); LABORATORY mice; IKAROS transcription factors; T cells; ANTIGENS
- Publication
Experimental Dermatology, 2015, Vol 24, Issue 7, p554
- ISSN
0906-6705
- Publication type
Article
- DOI
10.1111/exd.12711