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- Title
Ubiquitin-conjugated degradation of golden 2-like transcription factor is mediated by CUL4- DDB1-based E3 ligase complex in tomato.
- Authors
Tang, Xiaofeng; Miao, Min; Niu, Xiangli; Zhang, Danfeng; Cao, Xulv; Jin, Xichen; Zhu, Yunye; Fan, Youhong; Wang, Hongtao; Liu, Ying; Sui, Yuan; Wang, Wenjie; Wang, Anquan; Xiao, Fangming; Giovannoni, Jim; Liu, Yongsheng
- Abstract
CULLIN4- RING ubiquitin ligases ( CRL4s) as well as their targets are fundamental regulators functioning in many key developmental and stress responses in eukaryotes. In tomato ( Solanum lycopersicum), molecular cloning has revealed that the underlying genes of natural spontaneous mutations high pigment 1 ( hp1), high pigment 2 ( hp2) and uniform ripening ( u) encode UV- DAMAGED DNA BINDING PROTEIN 1 ( DDB1), DE- ETIOLATED 1 ( DET1) and GOLDEN 2- LIKE ( GLK2), respectively. However, the molecular basis of the opposite actions of tomato GLK2 vs CUL4- DDB1- DET1 complex on regulating plastid level and fruit quality remains unknown., Here, we provide molecular evidence showing that the tomato GLK2 protein is a substrate of the CUL4- DDB1- DET1 ubiquitin ligase complex for the proteasome degradation., Sl GLK2 is degraded by the ubiquitin-proteasome system, which is mainly determined by two lysine residues (K11 and K253). Sl GLK2 associates with the CUL4- DDB1- DET1 E3 complex in plant cells. Genetically impairing CUL4, DDB1 or DET1 results in a retardation of Sl GLK2 degradation by the 26S proteasome., These findings are relevant to the potential of nutrient accumulation in tomato fruit by mediating the plastid level and contribute to a deeper understanding of an important regulatory loop, linking protein turnover to gene regulation.
- Subjects
UBIQUITIN ligases; TRANSCRIPTION factors; GENETIC engineering of tomatoes; GENETIC regulation; PLANT proteins
- Publication
New Phytologist, 2016, Vol 209, Issue 3, p1028
- ISSN
0028-646X
- Publication type
Article
- DOI
10.1111/nph.13635