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- Title
Genomic Characterization of β-Glucuronidase-Positive Escherichia coli O157:H7 Producing Stx2a.
- Authors
Yoshitoshi Ogura; Kazuko Seto; Yo Morimoto; Keiji Nakamura; Sato, Mitsuhiko P.; Yasuhiro Gotoh; Takehiko Itoh; Atsushi Toyoda; Makoto Ohnishi; Tetsuya Hayashi; Ogura, Yoshitoshi; Seto, Kazuko; Morimoto, Yo; Nakamura, Keiji; Gotoh, Yasuhiro; Itoh, Takehiko; Toyoda, Atsushi; Ohnishi, Makoto; Hayashi, Tetsuya
- Abstract
Among Shiga toxin (Stx)-producing Escherichia coli (STEC) O157:H7 strains, those producing Stx2a cause more severe diseases. Atypical STEC O157:H7 strains showing a β-glucuronidase-positive phenotype (GP STEC O157:H7) have rarely been isolated from humans, mostly from persons with asymptomatic or mild infections; Stx2a-producing strains have not been reported. We isolated, from a patient with bloody diarrhea, a GP STEC O157:H7 strain (PV15-279) that produces Stx2a in addition to Stx1a and Stx2c. Genomic comparison with other STEC O157 strains revealed that PV15-279 recently emerged from the stx1a/stx2c-positive GP STEC O157:H7 clone circulating in Japan. Major virulence genes are shared between typical (β-glucuronidase-negative) and GP STEC O157:H7 strains, and the Stx2-producing ability of PV15-279 is comparable to that of typical STEC O157:H7 strains; therefore, PV15-279 presents a virulence potential similar to that of typical STEC O157:H7. This study reveals the importance of GP O157:H7 as a source of highly pathogenic STEC clones.
- Subjects
ESCHERICHIA coli; VEROCYTOTOXINS; ESCHERICHIA coli toxins; PATHOGENIC microorganisms; ENTEROBACTERIACEAE; METABOLISM; BACTERIAL toxins; COMPARATIVE studies; DNA; ESCHERICHIA coli diseases; BIOLOGICAL evolution; GENETIC polymorphisms; GENOMES; GLYCOSIDASES; RESEARCH methodology; MEDICAL cooperation; RESEARCH; MICROBIAL virulence; BIOINFORMATICS; GENOMICS; EVALUATION research; MITOMYCINS
- Publication
Emerging Infectious Diseases, 2018, Vol 24, Issue 12, p2219
- ISSN
1080-6040
- Publication type
journal article
- DOI
10.3201/eid2412.180404