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- Title
Usefulness of Published PCR Primers in Detecting Human Rhinovirus Infection.
- Authors
Faux, Cassandra E.; Arden, Katherine E.; Lambert, Stephen B.; Nissen, Michael D.; Nolan, Terry M.; Chang, Anne B.; Sloots, Theo P.; Mackay, Ian M.
- Abstract
We conducted a preliminary comparison of the relative sensitivity of a cross-section of published human rhinovirus (HRV)-specific PCR primer pairs, varying the oligonucleotides and annealing temperature. None of the pairs could detect all HRVs in 2 panels of genotyped clinical specimens; >1 PCR is required for accurate description of HRV epidemiology.
- Subjects
RHINOVIRUSES; OLIGONUCLEOTIDES; TEMPERATURE; EPIDEMIOLOGY; VIRUS diseases; COMMUNICABLE diseases
- Publication
Emerging Infectious Diseases, 2011, Vol 17, Issue 2, p296
- ISSN
1080-6040
- Publication type
Article
- DOI
10.3201/eid1702.101123