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- Title
An AS-PCR assay for accurate genotyping of FAD2A/FAD2B genes in peanuts (Arachis hypogaea L.).
- Authors
H. T. Yu; W. Q. Yang; Y. Y. Tang; X. Z. Wang; Q. Wu; D. Q. Hu; C. T. Wang; S. L. Yu
- Abstract
FAD2A and FAD2B are homoeologous genes from A and B genomes in cultivated peanuts (Arachis hypogaea L.) encoding fatty acid desaturates which convert oleate to linoleate. To study the genetics of oleate and breed high oleate peanut cultivars, a simple allele specific-PCR (AS-PCR) protocol for the accurate genotyping of FAD2A/FAD2B was developed to discriminate the wild and mutant allele of both genes (FAD2A 448 G > A and FAD2B 441_442insA). The results may serve to develop a feasible procedure for producing highly desired high oleate peanut cultivars through hybridization.
- Subjects
PEANUT research; PLANT genomes; FATTY acids; OLEATES; LINOLEIC acid
- Publication
Grasas y Aceites, 2013, Vol 64, Issue 4, p395
- ISSN
0017-3495
- Publication type
Article
- DOI
10.3989/gya.118712