We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Dangshen (Codonopsis pilosula) Activates IGF-I and FGF-2 Pathways to Induce Proliferation and Migration Effects in RSC96 Schwann Cells.
- Authors
Chen, Hsien-Tung; Tsai, Ying-Lan; Chen, Yueh-Sheng; Jong, Guo-Ping; Chen, Wei-Kung; Wang, Hwai-Lee; Tsai, Fuu-Jen; Tsai, Chang-Hai; Lai, Tung-Yuan; Tzang, Bor-Show; Huang, Chih-Yang; Lu, Chung-Yen
- Abstract
This study evaluates the proliferative and migrative effects of dangshen on RSC96, Schwann cells. We investigated the molecular signaling pathways, which include: (1) survival signaling, IGFs-IGFIR-Akt-Bcl2 and proliferative signaling, cell cycle factors and MAPK pathways. (2) migrate and anti-scar signaling, FGF-2-uPA-MMPs. After treatment with different concentrations (20 μg/ml, 40 μg/ml, 60 μg/ml, 80 μg/ml, and 100 μg/ml) of dangshen. We observed a dose dependent proliferative effect using PCNA Western blotting assay, MTT assay and the wound healing test. We also found that dangshen stimulates the protein expressions of IGF-I pathway regulators, cell cycle controlling proteins and excites the MAPK signaling pathway regulators ERK and P38. Dangshen even stimulates the FGF-2-uPA-MMP 9 migration pathway in RSC 96 Schwann cells. Using MAPK chemical inhibitors, U0126, SB203580, and SP600125, the proliferative effects of dangshen on RSC 96 cells were identified to be ERK- and P38- dependent. Based on these results, applying an appropriate dose of dangshen with biomedical materials would be a potential approach for enhancing neuron regeneration.
- Subjects
BIOPHYSICS; DOSE-response relationship in biochemistry; HERBAL medicine; RESEARCH methodology; MEDICINAL plants; CHINESE medicine; RESEARCH funding; TISSUE culture; WESTERN immunoblotting; WOUND healing; PLANT extracts; PHARMACODYNAMICS
- Publication
American Journal of Chinese Medicine, 2010, Vol 38, Issue 2, p359
- ISSN
0192-415X
- Publication type
Article
- DOI
10.1142/S0192415X10007907