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- Title
Female ovarian hormone- Estriol's proconvulsant effect on kainic acid induced seizures in mice followed by drosophila system model.
- Authors
Ahmad, Aakifa; Najmi, Abdul Kalam; Alam, Md. Naushad
- Abstract
The effect of estriol would be evaluated for its effect on Drosophila fly model and kindling model of epileptogenesis in mice followed by evaluation on kindling induced changes in cognitive and motor function. Seizures in drosophila and man have several similarities and the utility of the fruit fly as a genetic model system for studying human seizures disorder and seizure susceptibility has clearly been demonstrated (Glasscock et al.,2005). Kindling was induced on every alternate day for duration of 45 days and treatment with kianic acid was given at dose ranging from 15 to 30 mg/kg body weight intraperitoneal (i.p) and estriol was also administered at dosage of 0.005 and 0.1 mg/kg through i.p. After induction of kindling the seizure severity was recorded and further percentage incidence of animals kindled at the end of 45 days was also recorded. Spatial learning and cognitive alterations was assessed by Morris water test (MWT) and spontaneous alternation behavior (SAB) while motor function was assessed by grip strength meter. Drosophila fly of either sex and 3-4 days old unmated fly were used. Drosophila model, optimized for screening of drugs dissolved in DMSO, was used. Thirty flies were housed in each treatment vial. Three to four days old unmated male flies was treated with 8 mg/ml KA containing media for required time duration. Estriol enlarged the rate of kindling in both the sexes of mice at great rate. It further enhanced the percentage of incidence in seizures. Decline in the grip strength, spontaneous alteration behavior and morris water was observed following KA-kindling in pre-treated estriol groups of mice both the sexes. A regimen for drosophila fly model where it was treated with kainic acid and resulted in increases as well as decrease of climbing speed for adult Drosophila fly. Clomiphene produced kindlling within 2 weeks of dosage at 0.9 mg/kg and study established that estriol is a powerful proconvulsant drug. As higher organism has more colossal complexity thats why drosophila fly model is favored. This model is convenient to use and it is very relevant too.
- Subjects
KAINIC acid; DROSOPHILA; FRUIT fly control; EPILEPSY; ESTRIOL
- Publication
International Journal of Pharmacy & Life Sciences, 2018, Vol 9, Issue 7, p5844
- ISSN
0976-7126
- Publication type
Article