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- Title
Chemical Synthesis of K48-Linked Diubiquitin by Incorporation of a Lysine-Linked Auxiliary Handle.
- Authors
Xie, Rui-Long; Xu, Ling; Li, Jia-Bin; Chu, Guo-Chao; Wang, Tao; Huang, Yi-Chao; Li, Yi-Ming
- Abstract
We synthesized a lysine-linked auxiliary-derived building block, ϵ- N-2-mercapto-1-(2,4-dimethoxyphenyl)ethyl lysine, in 20 % isolated yield over a six-step route. The new building block is well compatible with Fmoc solid-phase peptide synthesis (SPPS) and hydrazide-based native chemical ligation (NCL). Furthermore, we successfully incorporated the new lysine-linked auxiliary handle into a protein segment and performed the chemical synthesis of K48-linked diubiquitin with stepwise NCL.
- Subjects
CHEMICAL synthesis; LYSINE synthesis; PEPTIDE synthesis; LIGATION reactions; UBIQUITINATION
- Publication
European Journal of Organic Chemistry, 2016, Vol 2016, Issue 15, p2665
- ISSN
1434-193X
- Publication type
Article
- DOI
10.1002/ejoc.201600292