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- Title
An Optically Controlled 3D Cell Culturing System.
- Authors
Ishii, Kelly S.; Wenqi Hu; Namekar, Swapnil A.; Ohta, Aaron T.
- Abstract
A novel 3D cell culture system was developed and tested. The cell culture device consists of a microfluidic chamber on an optically absorbing substrate. Cells are suspended in a thermoresponsive hydrogel solution, and optical patterns are utilized to heat the solution, producing localized hydrogel formation around cells of interest. The hydrogel traps only the desired cells in place while also serving as a biocompatible scaffold for supporting the cultivation of cells in 3D. This is demonstrated with the trapping of MDCK II and HeLa cells. The light intensity from the optically induced hydrogel formation does not significantly affect cell viability.
- Subjects
THREE-dimensional imaging; CELL culture; OPTICAL images; MICROFLUIDICS; COLLOIDS; SOLUTION (Chemistry)
- Publication
Advances in OptoElectronics, 2011, p1
- ISSN
1687-563X
- Publication type
Article
- DOI
10.1155/2011/253989