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- Title
Immunohistochemical expression of P53 in oral carcinogenesis.
- Authors
Garg, Shivani; Sandhu, Simarpreet Virk; Kaur, Preetinder; Bhullar, Ramampreet Kaur; Bhandari, Rajat; Singla, Kartesh
- Abstract
Context/Background: Carcinogenesis is a multistep process resulting in transformation of a normal cell to a malignant cell. Potentially malignant disorders (PMDs) carry an increased risk for malignant transformation in the oral cavity. Almost 33% of oral PMDs will eventually evolve into invasive oral squamous cell carcinoma (OSCC) over a 10-year interval, but no reliable histopathological parameters have been identified that predict their potential for subsequent transformation. Aim: The aim of this study was to evaluate predictive capacity of p53 to identify lesions at high risk of progression to invasive carcinoma. Materials and Methods: The retrospective study was conducted on 30 formalin-fixed paraffin-embedded tissues comprising 10 each of hyperplastic tissues (HPTs), PMDs and OSCC retrieved from the archives of our department. Immunohistochemical analysis was performed by using primary monoclonal antibody targeted against p53. The positivity of p53 cell count was assessed in the nucleus. Results: It was found that p53 positive cellcounts were more in OSCC as compared to PMDs and HPTs. The expression of p53 in HPTs was mainly localized to basal epithelial cells; whereas in PMDs and OSCC it involves basal and suprabasal epithelial cells. In OSCC the expression was found throughout the full thickness of epithelium (suprabasal). Conclusion: The expression of p53 above the basal layer could be an early event in oral carcinogenesis and an indicator of a developing carcinoma proceeding morphological tissue alterations.
- Subjects
CARCINOGENESIS; ORAL cancer; CANCER cells
- Publication
Journal of Oral & Maxillofacial Pathology (0973029X), 2014, Vol 18, pS42
- ISSN
0973-029X
- Publication type
Abstract