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- Title
Determining the geometry of oligomers of the human epidermal growth factor family on cells with <10 nm resolution.
- Authors
Needham, Sarah R.; Zanetti-Domingues, Laura C.; Scherer, Kathrin M.; Hirsch, Michael; Rolfe, Daniel J.; Roberts, Selene K.; Martin-Fernandez, Marisa L.; Clarke, David T.; Tynan, Christopher J.
- Abstract
There is a limited range of methods available to characterize macromolecular organization in cells on length scales from 5-50 nm. We review methods currently available and show the latest results from a new singlemolecule localization-based method, fluorophore localization imaging with photobleaching (FLImP), using the epidermal growth factor (EGF) receptor (EGFR) as an example system. Our measurements show that FLImP is capable of achieving spatial resolution in the order of 6 nm.
- Subjects
EPIDERMAL growth factor receptors; OLIGOMERS; GEOMETRY; MACROMOLECULES; SINGLE molecules
- Publication
Biochemical Society Transactions, 2015, Vol 43, Issue 3, p309
- ISSN
0300-5127
- Publication type
Article
- DOI
10.1042/BST20140318