We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Fast and Efficient Mouse Pluripotency Reprogramming Using a Chemically-Defined Medium.
- Authors
Huang, Junju; Yang, Xuejie; Wang, Jie; Wu, Haoyu; Pei, Duanqing; Chen, Jiekai
- Abstract
The reprogramming of somatic cells to obtain induced pluripotent stem cells (iPSCs) is an important biological and medical breakthrough, providing important applications for fields such as regenerative medicine and disease modeling. However, this promising technology is damped due to its low efficiency and slow kinetics. Therefore, we generated a practical workflow to rapidly and efficiently induce iPSCs from mouse embryonic fibroblasts (MEFs) using iCD1 (iPS chemically-defined medium 1). This protocol can easily be implemented in a standard cell culture laboratory and be applied to cell fate research.
- Subjects
PLURIPOTENT stem cells; SOMATIC cells; REGENERATIVE medicine; FIBROBLASTS; CELL culture; TRANSCRIPTION factors
- Publication
Methods & Protocols, 2022, Vol 5, Issue 2, pN.PAG
- ISSN
2409-9279
- Publication type
Article
- DOI
10.3390/mps5020028