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- Title
Responses of Gut Microbiota and Glucose and Lipid Metabolism to Prebiotics in Genetic Obese and Diet-Induced Leptin-Resistant Mice.
- Authors
Everard, Amandine; Lazarevic, Vladimir; Derrien, Muriel; Girard, Myriam; Muccioli, Giulio M.; Neyrinck, Audrey M.; Possemiers, Sam; Van Holle, Ann; François, Patrice; de Vos, Willem M.; Delzenne, Nathalie M.; Schrenzel, Jacques; Cani, Patrice D.
- Abstract
OBJECTIVE--To investigate deep and comprehensive analysis of gut microbial communities and biological parameters after prebiotic administration in obese and diabetic mice. RESEARCH DESIGN AND METHODS--Genetic (ob/ob) or diet-induced obese and diabetic mice were chronically fed with prebiotic-enriched diet or with a control diet. Extensive gut microbiota analyses, including quantitative PCR, pyrosequencing of the 16S rRNA, and phylogenetic microarrays, were performed in ob/ob mice. The impact of gut microbiota modulation on leptin sensitivity was investigated in diet-induced leptin-resistant mice. Metabolic parameters, gene expression, glucose homeostasis, and enteroendocrine-related L-cell function were documented in both models. RESULTS--In ob/ob mice, prebiotic feeding decreased Firmicutes and increased Bacteroidetes phyla, but also changed 102 distinct taxa, 16 of which displayed a > 10-fold change in abundance. In addition, prebiotics improved glucose tolerance, increased L-cell number and associated parameters (intestinal proglucagon mRNA expression and plasma glucagon-like peptide-1 levels), and reduced fat-mass development, oxidative stress, and low-grade inflammation. In high fat--fed mice, prebiotic treatment improved leptin sensitivity as well as metabolic parameters. CONCLUSIONS--We conclude that specific gut microbiota modulation improves glucose homeostasis, leptin sensitivity, and target enteroendocrine cell activity in obese and diabetic mice. By profiling the gut microbiota, we identified a catalog of putative bacterial targets that may affect host metabolism in obesity and diabetes.
- Subjects
LIPID metabolism; METABOLIC disorders; GLUCOSE metabolism; OBESITY; PREBIOTICS; MICE; OXIDATIVE stress
- Publication
Diabetes, 2011, Vol 60, Issue 11, p2775
- ISSN
0012-1797
- Publication type
Article
- DOI
10.2337/db11-0227