We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Lorentzian-Corrected Apparent Exchange-Dependent Relaxation (LAREX) Ω-Plot Analysis—An Adaptation for qCEST in a Multi-Pool System: Comprehensive In Silico, In Situ, and In Vivo Studies.
- Authors
Radke, Karl Ludger; Wilms, Lena Marie; Frenken, Miriam; Stabinska, Julia; Knet, Marek; Kamp, Benedikt; Thiel, Thomas Andreas; Filler, Timm Joachim; Nebelung, Sven; Antoch, Gerald; Abrar, Daniel Benjamin; Wittsack, Hans-Jörg; Müller-Lutz, Anja
- Abstract
Based on in silico, in situ, and in vivo studies, this study aims to develop a new method for the quantitative chemical exchange saturation transfer (qCEST) technique considering multi-pool systems. To this end, we extended the state-of-the-art apparent exchange-dependent relaxation (AREX) method with a Lorentzian correction (LAREX). We then validated this new method with in situ and in vivo experiments on human intervertebral discs (IVDs) using the Kendall-Tau correlation coefficient. In the in silico experiments, we observed significant deviations of the AREX method as a function of the underlying exchange rate (kba) and fractional concentration (fb) compared to the ground truth due to the influence of other exchange pools. In comparison to AREX, the LAREX-based Ω-plot approach yielded a substantial improvement. In the subsequent in situ and in vivo experiments on human IVDs, no correlation to the histological reference standard or Pfirrmann classification could be found for the fb (in situ: τ = −0.17 p = 0.51; in vivo: τ = 0.13 p = 0.30) and kba (in situ: τ = 0.042 p = 0.87; in vivo: τ = −0.26 p = 0.04) of Glycosaminoglycan (GAG) with AREX. In contrast, the influence of interfering pools could be corrected by LAREX, and a moderate to strong correlation was observed for the fractional concentration of GAG for both in situ (τ = −0.71 p = 0.005) and in vivo (τ = −0.49 p < 0.001) experiments. The study presented here is the first to introduce a new qCEST method that enables qCEST imaging in systems with multiple proton pools.
- Subjects
MAGNETIZATION transfer; INTERVERTEBRAL disk; IMAGING systems; FOREIGN exchange rates; MAGNETIC resonance imaging
- Publication
International Journal of Molecular Sciences, 2022, Vol 23, Issue 13, p6920
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms23136920