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- Title
Platelet-Reactive Antibodies in Patients after Ischaemic Stroke—An Epiphenomenon or a Natural Protective Mechanism.
- Authors
Park, Young Eun; Penumarthy, Rushi; Sun, Paul P.; Kang, Caroline Y.; Morel-Kopp, Marie-Christine; Downing, Jonathan; Green, Taryn N.; Immanuel, Tracey; Ward, Christopher M.; Young, Deborah; During, Matthew J.; Barber, P. Alan; Kalev-Zylinska, Maggie L.
- Abstract
Ischaemic brain damage induces autoimmune responses, including the production of autoantibodies with potential neuroprotective effects. Platelets share unexplained similarities with neurons, and the formation of anti-platelet antibodies has been documented in neurological disorders. The aim of this study was to investigate the presence of anti-platelet antibodies in the peripheral blood of patients after ischaemic stroke and determine any clinical correlations. Using a flow cytometry-based platelet immunofluorescence method, we detected platelet-reactive antibodies in 15 of 48 (31%) stroke patients and two of 50 (4%) controls (p < 0.001). Western blotting revealed heterogeneous reactivities with platelet proteins, some of which overlapped with brain proteins. Stroke patients who carried anti-platelet antibodies presented with larger infarcts and more severe neurological dysfunction, which manifested as higher scores on the National Institutes of Health Stroke Scale (NIHSS; p = 0.009), but they had a greater recovery in the NIHSS by the time of hospital discharge (day 7 ± 2) compared with antibody-negative patients (p = 0.043). Antibodies from stroke sera reacted more strongly with activated platelets (p = 0.031) and inhibited platelet aggregation by up to 30.1 ± 2.8% (p < 0.001), suggesting the potential to interfere with thrombus formation. In conclusion, platelet-reactive antibodies can be found in patients soon after ischaemic stroke and correlate with better short-term outcomes, suggesting a potential novel mechanism limiting thrombosis.
- Subjects
NATIONAL Institutes of Health (U.S.); IMMUNOGLOBULINS; BLOOD platelet aggregation; STROKE; NEUROLOGICAL disorders; STROKE patients; PLATELET count; AUTOANTIBODIES
- Publication
International Journal of Molecular Sciences, 2020, Vol 21, Issue 21, p8398
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms21218398