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- Title
Immunomodulatory Protein from Nectria haematococca Induces Apoptosis in Lung Cancer Cells via the P53 Pathway.
- Authors
Wang, Jing-Jing; Wang, Yan; Hou, Lizhen; Xin, Fengjiao; Fan, Bei; Lu, Cong; Zhang, Lijing; Wang, Fengzhong; Li, Shuying
- Abstract
Our previous research has shown that a fungal immunomodulatory protein from Nectria haematococca (FIP-nha) possesses a wide spectrum of anti-tumor activities, and FIP-nha induced A549 apoptosis by negatively regulating the PI3K/Akt signaling pathway based on comparative quantitative proteomics. This study further confirmed that the anti-lung cancer activity of FIP-nha was significantly stronger than that of the reported LZ-8 and FIP-fve. Subsequently, 1H NMR-based metabolomics was applied to comprehensively investigate the underlying mechanism, and a clear separation of FIP-nha-treated and untreated groups was achieved using pattern recognition analysis. Four potential pathways associated with the anti-tumor effect of FIP-nha on A549 cells were identified, and these were mainly involved in glycolysis, taurine and hypotaurine metabolism, fructose and mannose metabolism, and glycerolipid metabolism. Metabolic pathway analysis demonstrated that FIP-nha could induce A549 cell apoptosis partly by regulating the p53 inhibition pathway, which then disrupted the Warburg effect, as well as through other metabolic pathways. Using RT-PCR analysis, FIP-nha-induced apoptosis was confirmed to occur through upregulation of p53 expression. This work highlights the possible use of FIP-nha as a therapeutic adjuvant for lung cancer treatment.
- Subjects
CANCER cells; LUNG cancer; FUNGAL proteins; ADJUVANT treatment of cancer; APOPTOSIS; METABOLOMICS
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 21, p5348
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20215348