We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Anti-Proliferative and Pro-Apoptotic Effects of Licochalcone A through ROS-Mediated Cell Cycle Arrest and Apoptosis in Human Bladder Cancer Cells.
- Authors
Hong, Su Hyun; Cha, Hee-Jae; Hwang-Bo, Hyun; Kim, Min Yeong; Kim, So Young; Ji, Seon Yeong; Cheong, JaeHun; Park, Cheol; Lee, Hyesook; Kim, Gi-Young; Moon, Sung-Kwon; Yun, Seok Joong; Chang, Young-Chae; Kim, Wun-Jae; Choi, Yung Hyun
- Abstract
Licochalcone A (LCA) is a chalcone that is predominantly found in the root of Glycyrrhiza species, which is widely used as an herbal medicine. Although previous studies have reported that LCA has a wide range of pharmacological effects, evidence for the underlying molecular mechanism of its anti-cancer efficacy is still lacking. In this study, we investigated the anti-proliferative effect of LCA on human bladder cancer cells, and found that LCA induced cell cycle arrest at G2/M phase and apoptotic cell death. Our data showed that LCA inhibited the expression of cyclin A, cyclin B1, and Wee1, but increased the expression of cyclin-dependent kinase (Cdk) inhibitor p21WAF1/CIP1, and increased p21 was bound to Cdc2 and Cdk2. LCA activated caspase-8 and -9, which are involved in the initiation of extrinsic and intrinsic apoptosis pathways, respectively, and also increased caspase-3 activity, a typical effect caspase, subsequently leading to poly (ADP-ribose) polymerase cleavage. Additionally, LCA increased the Bax/Bcl-2 ratio, and reduced the integrity of mitochondria, which contributed to the discharge of cytochrome c from the mitochondria to the cytoplasm. Moreover, LCA enhanced the intracellular levels of reactive oxygen species (ROS); however, the interruption of ROS generation using ROS scavenger led to escape from LCA-mediated G2/M arrest and apoptosis. Collectively, the present data indicate that LCA can inhibit the proliferation of human bladder cancer cells by inducing ROS-dependent G2/M phase arrest and apoptosis.
- Subjects
CELL cycle; CANCER cells; BLADDER cancer; APOPTOSIS; CYTOCHROME c
- Publication
International Journal of Molecular Sciences, 2019, Vol 20, Issue 15, p3820
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms20153820