We found a match
Your institution may have rights to this item. Sign in to continue.
- Title
Common microRNA–mRNA Interactions in Different Newcastle Disease Virus-Infected Chicken Embryonic Visceral Tissues.
- Authors
Jia, Yan-Qing; Wang, Xing-Long; Wang, Xiang-Wei; Yan, Chuan-Qi; Lv, Chang-Jie; Li, Xiao-Qin; Chu, Zhi-Li; Adam, Fathalrhman Eisa Addoma; Xiao, Sa; Zhang, Shu-Xia; Yang, Zeng-Qi
- Abstract
To investigate the roles and explore the altered expression of microRNAs (miRNAs) and mRNAs in chicken embryos in response to Newcastle disease virus (NDV) infection, deep sequencing was performed. Then, a conjoint analysis of small RNA-seq and mRNA-seq was performed to screen interactional miRNA–mRNA pairs during NDV infection. In total, 15 and 17 up- and downregulated miRNAs were identified that potentially targeted 4279 and 6080 mRNAs in NDV-infected chicken embryonic tissues, respectively; in addition, 595 upregulated and 480 downregulated mRNAs were identified. The conjoint analysis of the obtained data identified 1069 miRNA–mRNA pairs. Among these pairs, 130 pairs were related to immune or inflammatory responses. The relationship between gga-miR-203a and its target transglutaminase 2 (TGM2) was confirmed using a dual-luciferase reporter system and a real time quantitative polymerase chain reaction (RT-qPCR) assay. Overall, the discovery of miRNAs, mRNAs, and their potential pairing relationships, which may be involved in the regulation of NDV infection, will facilitate our understanding of the complex regulatory relationship between the host and the virus.
- Subjects
NEWCASTLE disease virus; VIRUS diseases in poultry; CHICKEN embryos; MICRORNA; MESSENGER RNA; TISSUES; DISEASES
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 5, p1291
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19051291